CI-302 High Pressure Diffuser


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Product Details

General information

The CI-302 High Pressure Diffuser is used when sampling pressurized air or gases with Climet particle counters or microbial air samplers.

Applying compressed air or gases directly into a particle counter or microbial sampler may cause damage to the instrument (i.e., the HEPA filter). Also, with particle counters it will also cause turbulence inside the sensor that produces false high counts, and will also result in gross under-sizing of particles. Connecting high pressure gases directly to a microbial air sampler without a diffuser will result in a substantial degradation of biological efficiencies due to an extremely high impaction velocity.

The CI-302 works by regulating the flow rate with an exhaust port to provide a path for the excess air pressure. This allows the particle counter to “pull in” and regulate the flow of air or gas into the instrument.

Special Calibration & Use

Common compressed gases used in sampling are air, nitrogen, argon, helium and carbon dioxide.  The mass flow is affected by the density of the gases being sampled.  Air and nitrogen have virtually the same density.  However, argon, helium, and carbon dioxide have different densities that shift the air flow.  When sampling argon, helium and carbon dioxide it is highly recommended to have the particle counter or microbial sampler’s flow rate calibrated for the particular gas being sampled.

Due to safety concerns, Climet does not recommend sampling oxygen.

Cleaning and Maintenance

The CI-302 is compliant with ISO 8573-4, and is autoclavable.

The CI-302 has no moving parts and does not need calibration. Over time it is possible that particles may build up on surfaces within the diffuser, and require cleaning. It is recommended that the diffuser be returned to the factory if counts become elevated over time, suggesting that the diffuser needs to be cleaned. As long as counts remain consistently at historical levels, cleaning the diffuser is not indicated.


Interoperability is assured with all Climet microbial samplers, and particle counters (excluding the RS version).

When used with Climet microbial samplers, a High Pressure Diffuser Adapter Head (p/n: 01210109) will be required.

The low pressure end of the diffuser has a dual size outlet fitting.  This allows 1/2″ or 3/8″ tubing to be connected between the CI-302, and the particle counter inlet or microbial adapter inlet.  On the high pressure end, a 1/4″ tube connection is compatible with a number of fittings (default VCO).

Configuration & Best Practices

As the flow rate of the particle counter or microbial sampler increases, the narrower the operating range of the diffuser. This may be important when sampling gases with various pressures.  Additionally, the following must be taken into consideration during setup:

The inside diameter of the high pressure gas line can be critical when sampling with 100 LPM. Due to flow restriction, the minimum diameter of the High Pressure Gas Line (see image below) should be 1/4 inch or larger (ID). If piping is less than 1/4″, we recommend testing with 1 CFM or 50 LPM particle counter or microbial air sampler is recommended.
High pressure (80 psi and higher) increases gas friction inside transport piping causing particles to slough off the walls of the piping resulting in extremely high counts.  For these reasons we normally recommend regulating gases to under 80 psi, or sample with a lower flow rate particle counter.
Ten (10) feet of 3/8″ and 1/2″ Bev-A Line tubing is provided with each purchase.  This tubing is used to connect to the particle counter or microbial air sampler (i.e., low pressure end).  The minimum tubing length should be ten (10) feet in order to mitigate sonic waves and false counts.
A VCO fitting and Teflon valve (i.e., the VCO valve assembly , P/N 01276300)  is provide with each purchase. Installation of this is recommended to mitigate the generation of particles, and to provide a secure connection on the high pressure gas line. The Teflon valve is critical, as users of metal valves frequently require a minimum delay of 5 minutes (while gas is being expelled) in order to allow metal particles to pass and counts to return to nominal levels. Thus, metal valves are not recommended as it will frequently result in false high counts, wasted gas, and employee time.
To mitigate particle contamination of the high pressure diffuser and contamination of the sensor, to start sampling turn the valve on then start the particle counter. When sampling is complete, shut the particle counter off first then turn the value off.
When sampling, we recommend not trying to regulate pressure by hand, but rather fully open the pressure inlet (1/2 turn on the Teflon valve), and let the High Pressure Diffuser exhaust the excess compressed gas and do what it was intended to do – – otherwise you risk under sampling.



Step 1 Confirm the flow rate of the Climet particle counter or microbial sampler? This is referred to as the “Low Pressure End”. In this example, we will assume a 1 CFM particle counter.

Step 2 Confirm the pressure of the gas being sampled? This is referred to as the “High Pressure End”. You will want to determine the static gas pressure after the regulator and filter. Let’s assume 70 psi.

Step 3 Drop down to the blue rows, and select the row with the lowest number. In this example, the lowest number is 71.4

Step 4 Choose the part number (Far left column) on the blue row with lowest number. Again, in this example, the part number is CI-302-20. The last two digits of the part number are the orifice size inside the diffuser.  Too large an orifice will result in a waste of gas.




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